For separating the non-volatile mixtures, a special chromatography technique is used, i.e. Thin Layer chromatography. It works on the principle of separation in which the different components are adsorbed on the surface of adsorbent such as silica gel, alumina or cellulose. It is carried out on a sheet or plate of glass, metal or plastic which is covered with thin layer of solid adsorbent material. This thin layer of adsorbent is also called as stationary phase. Usually, silica gel is used as an adsorbent with the size ranging from 400 to 800 meshes for tlc different binding grades including silica gel g, silica gel f, silica gel gf 254, silica gel hf 254.
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